Stem Cells Characterization and Biomedical Importance...

Over the past decade, stem cell biology has been an area that has caused much controversy. Stem cells have the ability to differentiate into many different types of cells and therefore, advocates of stem cell research argue that the cells have various medical applications. On the other hand, opponents of stem cell research denounce the use of human embryos for research purposes, claiming that the embryos represent human lives and that experimentation with them and subsequent annihilation of them is the same as killing a living human being. Nevertheless, the potential uses for stem cells are endless and stem cells have the ability to completely change modern medical practices. Human embryonic stem cells (HESCs) are characterized by†¦show more content†¦Prior to 2001, the primary method used to isolate embryonic stem cell lines was to identify and segregate single colonies under a dissecting microscope. However, this process was time-consuming and costly and served to inhibit stem cell research. A new method has been developed that involves introducing a reporter gene into the embryonic stem cells and separating stem cell lines due to the genes effects (Eiges et al. 2001). The human embryonic stem cells were first transfected with enhanced green fluorescence protein (EGFP), under the control of the murine Rex-1 promoter. The cells with EGFP demonstrated high levels of GFP expression when in the undifferentiated state and therefore showed high levels of fluorescence. As the cells differentiated, the levels of GFP expression decrease along with the degree of fluorescence. The undifferentiated cells were then isolated from the culture using a Fluorescence Activated Cell Sorter (FACS) (Eiges et al. 2001). Furthermore, the pluripotent nature of the human embryonic stem cells was verified due to the results obtained using this new method. Since the fluorescence of the stem cells changed in some cases, it was clear that the human embryonic stem cells had differentiated. In addition, the ability of the stem cells to form embryoid bodies (EBs), Irregularly shaped clumps of cellular structures that contain tissue from all three of theShow MoreRelatedThe Importance Of A 3d Supportive Structure3116 Words   |  13 PagesAs mentioned before, in tissue regeneration is of crucial importance the use of a 3D supportive structure because when cells are seeded into these scaffolds enables the proliferation of cells and chondrogenic differentiation; and with it the three-dimensional tissue formation. Scaffolds must be able to withstand physiological loading until sufficient tissue regeneration occurs. Moreover, the material must be sufficiently porous to allow for effective transport of nutrients. Finally, it should beRead More_x000C_Introduction to Statistics and Data Analysis355457 Words   |  1422 PagesBurgers 64 Activity 2.4 Video Games and Pain Management 65 Graphing Calculator Explorations 69 3 Graphical Methods for Describing Data 75 3.1 Displaying Categorical Data: Comparative Bar Charts and Pie Charts 76 3.2 Displaying Numerical Data: Stem-and-Leaf Displays 87 vii viii ââ€"   Contents 3.3 Displaying Numerical Data: Frequency Distributions and Histograms 97 3.4 Displaying Bivariate Numerical Data 117 3.5 Interpreting and Communicating the Results of Statistical Analyses 127Read MoreQuality Improvement328284 Words   |  1314 Pagesfundamental concepts of designed experiments and introduces factorial and fractional factorial designs, with particular emphasis on the two-level system of designs. These designs are used extensively in the industry for factor screening and process characterization. Although the treatment of the subject is not extensive and is no substitute for a formal course in experimental design, it will enable the reader to appreciate more sophisticated examples of experimental design. Chapter 14 introduces response